Isolation and Identification of volatiles compounds from Rice Weeil Weevil , Sitophilus oryzae (coleptera Coleoptera : Curculionidae) in Iran with Solid Phase Micro Extraction (SPME) and Headspace Chromatography

Rice weevil Sitophilus oryzae (L),cosmopolit Insect which today is scattered in all parts of the world .This pest often makes much damage on rice and other stored grains.Pesticides residue cause restriction use of these chemicals in some places such as warehouses and store locations. As Itegrated Pest Management is new strategy for pest control,Identification of specific volatiles compounds is necessary. Some studies have shown that pheromones compounds of a specific species could have some changes in different environments or nutritional conditions and this is effective at the efficacy of pheromones. This study were conducted to isolation and determination of volatile chemicals by SPME-HS Chromatography. Test Insects growed up in 27[plus or minus]1 Celsius degree temperature and 65[plus or minus]5 percent relative humidity. Volatile extraction were done in four different times duration with three replicates for each of them and virgin weevils used with median age 1-10 days after transferring them to sterile glass vials. SPME fibers contacted to head space of the vials then analyzed by (Gas Chromatography/Mass Spectrometry(GC/MS) system. Detected compounds checked by Willy and NIST database software. Results showed 16 different compounds .most of them were similar with Detected compounds by other researchers but some of them were new. This difference may be for different geographical or nutritional condition, or may be for difference in extraction time. More study is needed for better determination and application of these compounds for IPM.