02235nas a2200229   4500008004100000020001400041245011600055210006900171260000800240300001200248490000700260520141800267653002701685653002801712100001601740700002101756700001901777700002801796700002201824700002401846856013501870       2011                        eng d  a0095-362800aPresence and Diversity of Streptomyces in Dendroctonus and Sympatric Bark Beetle Galleries Across North America0 aPresence and Diversity of Streptomyces in Dendroctonus and Sympa  cMay  a759-7680 v613 aRecent studies have revealed several examples of intimate associations between insects and Actinobacteria, including the Southern Pine Beetle Dendroctonus frontalis and the Spruce Beetle Dendroctonus rufipennis. Here, we surveyed Streptomyces Actinobacteria co-occurring with 10 species of Dendroctonus bark beetles across the United States, using both phylogenetic and community ecology approaches. From these 10 species, and 19 other scolytine beetles that occur in the same trees, we obtained 154 Streptomyces-like isolates and generated 16S sequences from 134 of those. Confirmed 16S sequences of Streptomyces were binned into 36 distinct strains using a threshold of 0.2% sequence divergence. The 16S rDNA phylogeny of all isolates does not correlate with the distribution of strains among beetle species, localities, or parts of the beetles or their galleries. However, we identified three Streptomyces strains occurring repeatedly on Dendroctonus beetles and in their galleries. Identity of these isolates was corroborated using a house-keeping gene sequence (efTu). These strains are not confined to a certain species of beetle, locality, or part of the beetle or their galleries. However, their role as residents in the woodboring insect niche is supported by the repeated association of their 16S and efTu from across the continent, and also having been reported in studies of other subcortical insects.10aDendroctonus frontalis10aDendroctonus rufipennis1 aHulcr, Jiri1 aAdams, Aaron, S.1 aRaffa, Kenneth1 aHofstetter, Richard, W.1 aKlepzig, Kier, D.1 aCurrie, Cameron, R.  uhttps://weevil.myspecies.info/presence-and-diversity-streptomyces-dendroctonus-and-sympatric-bark-beetle-galleries-across-north-am02333nas a2200193   4500008004500000020001400045245010500059210006900164260001100233300001200244490000700256520163200263653002801895100002401923700002001947700002201967700002301989856012702012       2008                        Engldsh    a0046-225X00aMultipartite symbioses among fungi, mites, nematodes, and the spruce beetle, Dendroctonus rufipennis0 aMultipartite symbioses among fungi mites nematodes and the spruc  cAugust  a956-9630 v373 aThe spruce beetle, Dendroctonus rufipennis, is an eruptive forest pest of significant economic and ecological importance. D. rufipennis has symbiotic associations with a number of microorganisms, especially the ophiostomatoid fungus Leptographium abietinum. The nature of this interaction is only partially understood. Additionally, mite and nematode associates can mediate bark beetle-fungal interactions, but this has not yet been studied for spruce beetles. In this study, we found eight mite species associated with spruce beetles: Tarsonemus ips, T. endophloeus, Histiogaster arborsignis, Dendrolaelaps quadrisetus, Proctolaelaps hytricoides, Trichouropoda alascae, T. n. sp. nr dalarenaensis, and Urobovella n. sp 767. The most prevalent species was H. arborsignis. In addition, 75% of beetles examined carried nematodes, with six species represented. These included a new species of Parasitorhabditis, Ektaphelenchus obtusus, Bursaphelenchus n. sp. 727, Aphelenchoides n. sp., Panagrolaimus sp., and Mykoletzkya ruminis. H. arborsignis showed strong feeding and oviposition preferences for L. abietinum among four fungal species tested in laboratory assays. Information on our attempts to culture the various nematode species collected from D. rufipennis is also provided. Bursaphelenchus were cultured from D. rufipennis nematangia plated on agar containing L. abietinum but not sterile agar, Thus, L. abietinum plays an important role in these gallery communities, affecting the tree-killing bark beetle, its phoretic mites, and nematodes. These data add to our understanding of bark beetle-microorganism interactions.10aDendroctonus rufipennis1 aCardoza, Yasmin, J.1 aMoser, John, C.1 aKlepzig, Kier, D.1 aRaffa, Kenneth, F.  uhttps://weevil.myspecies.info/multipartite-symbioses-among-fungi-mites-nematodes-and-spruce-beetle-dendroctonus-rufipennis