02077nas a2200181   4500008004100000020001400041245018300055210006900238260000900307300001200316490000800328520134500336653001701681100002301698700001801721700002101739856013501760       2010                        eng d  a0022-049300aMolecular Identification of Larval Stages of Otiorhynchus (Coleoptera: Curculionidae) Species Based on Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis0 aMolecular Identification of Larval Stages of Otiorhynchus Coleop  cJune  a898-9070 v1033 aA couple of different members of the coleopteran genus Otiorhynchus (Coleoptera: Curculionidae) are becoming increasingly important as pests of nursery and ornamental plants in global horticulture. Although adult weevils are morphologically distinguishable by skilled personnel, high potential for misidentification is given for cryptic larval stages. For developing and applying efficient pest management strategies the determination of the respective species is however a prerequisite, because each species may have a different phenology or a varying susceptibility to pesticides. Here, we report on the development of a diagnostic polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method for differentiation among 16 Otiorhynchus and seven other weevil species independent of their developmental stage. An [approximately]780-bp fragment of the mitochondrial cytochrome oxidase subunit II was amplified and subsequently digested with at most four restriction enzymes generating species-specific fragment patterns. The assay was validated on a total of 127 individuals and the obtained fragment patterns correctly identified 23 different weevil species. The PCR-RFLP method reported here is cost-effective, robust, and fast and could be used in the future by plant protection services for diagnostic purposes.10aOtiorhynchus1 aHirsch, Jacqueline1 aSprick, Peter1 aReineke, Annette  uhttps://weevil.myspecies.info/molecular-identification-larval-stages-otiorhynchus-coleoptera-curculionidae-species-based-polymeras01311nas a2200181   4500008004100000020001400041245018400055210006900239260001600308300001000324490000700334520056800341653002700909100001700936700002300953700001800976856013500994       2010                        eng d  a1439-465000aBiology and molecular determination of Pachyrhinus lethierryi (Desbrochers, 1875) with description of the mature larva and pupa (Coleoptera, Curculionidae, Entiminae: Polydrusini)0 aBiology and molecular determination of Pachyrhinus lethierryi De  c15 December  a80-950 v113 aOriginal range, spreading, life-cycle, egg-laying behaviour, habitat and host plants of Pachyrhinus lethierryi (Desbrochers, 1875) and its larvae were studied. The mature larva and the pupa are described, illustrated and compared to immature life stages of Pachyrhinus mustela (Herbst, 1797) for the first time. In addition to the morphological description, a fragment of the cytochrome oxidase subunit II (COII) was analysed and a diagnostic molecular marker for the identification of Pachyrhinus lethierryi, independent of the developmental stage, was generated10aPachyrhinus lethierryi1 aGosik, Rafal1 aHirsch, Jacqueline1 aSprick, Peter  uhttps://weevil.myspecies.info/biology-and-molecular-determination-pachyrhinus-lethierryi-desbrochers-1875-description-mature-larva