01960nas a2200169   4500008004500000020001400045245012800059210006900187260001200256300001400268490000700282520129700289653002701586100001601613700002601629856013501655       2008                        Engldsh    a0046-225X00aEvaluation of reproductive compatibility of interstrain matings among plum cuculio populations in the eastern United States0 aEvaluation of reproductive compatibility of interstrain matings   cOctober  a1208-12130 v373 aReproductive compatibility of interstrain matings in plum curculio, Conotrachelus nenuphar (Herbst), was studied during the summers of 2004 and 2006. There was a unidirectional incompatibility in the cross-matings along a rough north to south transect of the range of plum curculio (4 x 4 two-factorial design). There was significantly lower fertility in West Virginia males mated with New York and Virginia females (40 and 29%, respectively), and Florida males had a significantly lower fertility with Virginia and West Virginia females (46 and 37%, respectively), but Florida females were compatible with males of all four populations. Three northern populations were compatible with each other in all cross-mating combinations (New York, Massachusetts, and New Jersey; 3 x 3 two-factorial design). There was a unidirectional reproductive incompatibility in the combination of New Jersey males with Florida females (47%) and a bi-directional incompatibility between Florida and West Virginia reciprocal cross-mating (26 and 21%, respectively). The pattern of the reproductive incompatibility among geographic populations could help us to distinguish plum curculio strains and to clarify their strain distribution range to enrich our knowledge on their population ecology and biosystematics.10aConotrachelus nenuphar1 aZhang, Xing1 aPfeiffer, Douglas, C.  uhttps://weevil.myspecies.info/evaluation-reproductive-compatibility-interstrain-matings-among-plum-cuculio-populations-eastern-uni02518nas a2200193   4500008004100000020001400041245013000055210006900185260001000254300001200264490000700276520179900283653002702082100001602109700002202125700001602147700002602163856013502189       2010                        eng d  a0046-225X00aAnalysis of Wolbachia Strains Associated With Conotrachelus nenuphar (Coleoptera: Curculionidae) in the Eastern United States0 aAnalysis of Wolbachia Strains Associated With Conotrachelus nenu  cApril  a396-4050 v393 aWe studied the distribution patterns of Wolbachia infection associated with plum curculio strains in eight states of the eastern United States. The presence of the Wolbachia-specific gene wsp identified infections of this endosymbiont in 97.8% of the 93 samples tested. Three distinct Wolbachia strains were identified. The strains wCnel (593 bp) and wCne2 (593 bp) were 97% identical, and their sequences were both 84% identical with wCne3 (590 bp). BLASTN searches through GenBank showed strong similarities between the wsp sequences of the three strains compared with Wolbachia sequenced from other hosts. Degree of similarity with sequences in other Wolbachia strains is discussed. Polymerase chain reaction-restriction fragment length polymorphism was used for superinfection detection. Of 93 samples, 15 (16.1%), 21 (22.6%), 19 (20.4%), and 36 (38.7%) samples were infected by wCnel, wCne2, wCnel + 2, and wCne3, respectively. Only two (2.2%) samples had no infection. The wCne3 strain was always present as a single infection. Wolbachia strains approximate the distribution of plum curculio strains: northern strain infected with wCnel and wCne2 strains in supergroup B, and southern strain infected with wCne3 strain in supergroup A, with the mid-Atlantic region as the convergence area. Based on haplotype distribution of plum curculio mitochondrial cytochrome oxidase I, there was a closer relation of the mid-southern plum curculio clade to the far-southern clade than to the northern clade. However, Wolbachia symbionts in mid-southern plum curculio are more closely related to those in northern plum curculio than to those in far-southern plum curculio. The relationship of Wolbachia infection with reproductive incompatibility between plum curculio populations was also discussed.10aConotrachelus nenuphar1 aZhang, Xing1 aLuckhart, Shirley1 aTu, Zhijian1 aPfeiffer, Douglas, G.  uhttps://weevil.myspecies.info/analysis-wolbachia-strains-associated-conotrachelus-nenuphar-coleoptera-curculionidae-eastern-united02012nas a2200193   4500008004500000020001400045245012700059210006900186260001400255300001200269490000800281520128700289653002701576100001601603700001601619700002201635700002601657856013501683       2008                        Engldsh    a0013-874600aGenetic diversity of plum curculio (Coleoptera: Curculionidae) among geographical populations in the eastern United States0 aGenetic diversity of plum curculio Coleoptera Curculionidae amon  cSeptember  a823-8320 v1013 aThis study investigated the genetic diversity among plum curculio, Conotrachelus nenuphar (Herbst), populations from 11 different geographic locations by using partial sequences of mitochondrial cytochrome oxidase I (mtCOI) gene. Polymerase chain reaction (PCR) products were 863 bp. In total, 23 unique haplotypes were identified from 50 tested samples. Haplotypes G (n = 5), L (n =12), and T (n = 13) made up 60% of the 50 samples. Two distinct major clades were found in the phylogenetic analyses. Northern and southern clades were strongly supported (100/83 (MP/ ML) and 100/62, respectively) by bootstraps. The mid-southern subclade within the southern clade was also moderately supported by bootstraps (73/57, MP/ML). The results of analyses of molecular variance (AMOVA) are consistent with phylogenetic analysis. The results suggested that the northern and the southern clades correspond with northern strain and southern strain of plum curculio. The three-group model of AMOVA test may suggest that there is a transitional group existing between northern and southern groups. In this study, the mtCOI sequence was highly informative as a molecular marker in that it could distinguish C. nenuphar from northern and southern geographic locations in the eastern United States.10aConotrachelus nenuphar1 aZhang, Xing1 aTu, Zhijian1 aLuckhart, Shirley1 aPfeiffer, Douglas, G.  uhttps://weevil.myspecies.info/genetic-diversity-plum-curculio-coleoptera-curculionidae-among-geographical-populations-eastern-unit