@article {11287,
	title = {A modified method to visualize infection sites of spores of the entomopathogen Beauveria bassiana (Deuteromycotina: Hyphomycetes) on the exoskeleton of citrus root weevil Diaprepes abbreviatus (Coleoptera: Curculionidae) adults},
	journal = {Florida Entomologist},
	volume = {92},
	number = {4},
	year = {2009},
	month = {December},
	pages = {623-628},
	abstract = {Beauveria bassiana is a widespread entomopathogen which is infectious to a great variety of insects. A commercial preparation of this fungus was used to study its potential as a biocontrol agent of the citrus root weevil, Diaprepes abbreviatus. Laboratory reared adult weevils were placed in clean plastic bags with 0.05 g of powdered inoculum per bag, shaken for thirty seconds, and placed in holding cages. In order to determine the spore concentration, germination, and position of ingress, inoculated weevils were dipped in a collodion solution after 30 min, 6 h, 12 h, 18 h, and 30 h. Collodion peels from various areas of the exoskeleton were removed, stained with lactophenol cotton blue, and observed microscopically. At 12 h post-inoculation spores began to swell, and at 18 h close to 25\% germinated on all body parts except on the elytra. After 30 h, between 45\% and 75\% of the spores germinated, depending on the body part. Most active spore germination occurred around the eyes (75\%), followed by the ventral abdomen (65\%), the dorsal pronotum (60\%), scales (45\%), and dorsal surface of the elytra (7\%). Spores were washed from the surface of inoculated weevils with distilled water and plating onto potato dextrose agar. Spore density on the weevils{\textquoteright} surface was determined to be 15,815 [plus or minus] 479 spores initially after inoculation, and was reduced to 5,782 [plus or minus] 136 spores after 3 h.},
	keywords = {Diaprepes abbreviatus},
	isbn = {0015-4040},
	author = {Gillett-Kaufman, Jennifer L. and Kimbrough, James W.}
}
