@article {13359,
	title = {Molecular diagnostic for boll weevil (Coleoptera: Curculionidae) based on amplification of three species-specific microsatellites},
	journal = {Journal of Economic Entomology},
	volume = {102},
	number = {2},
	year = {2009},
	month = {April},
	pages = {759-766},
	abstract = {The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a serious pest of cultivated cotton, Gossypium hirsutum, L., in the Americas, and reinfestation of zones from which they have been eradicated is of perpetual concerti. Extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. For example, the congeneric pepper weevil, Anthonomus eugenii Cano, and other superficially similar weevils are attracted to components of the boll weevil lure or trap color. Although morphologically distinguish able by trained personnel, the potential for misidentification is compounded when captured weevils are dismembered or partially consumed by ants or ground beetles that sometimes feed on them in the traps. Because misidentification can have expensive consequences, a molecular diagnostic tool would be of great value to eradication managers. We demonstrate that a cocktail of three primer pairs in a single polymerase chain reaction (PCR) amplify species-specific microsatellites that unambiguously distinguish the boll weevil from three other weevil species tested, including pepper we evil; cranberry weevil, Anthonomus eugenii musculus Say; and pecan weevil, Curculio caryae Horn. However, it does not distinguish the boll weevil from the subspecific "thurberia" weevil. A universal internal transcribed spacer primer pair included in the cocktail cross-amplifies DNA from all species, serving as a positive control. Furthermore, the diagnostic primers amplified the target microsatellites from various boll weevil adult body parts, indicating that the PCR technology using the primer cocktail is sensitive enough to positively identify a boll weevil even when the body is partly degraded.},
	keywords = {Anthonomus grandis grandis},
	isbn = {0022-0493},
	author = {Kim, Kyung Seok and Szendre, Zsofia and Rodriguez-Saona, Cesar and Mulder, Phillip C., Jr. and Sappington, Thomas W.}
}
@article {8472,
	title = {Behavioral and Electrophysiological Responses of Listronotus maculicollis (Coleoptera: Curculionidae) to Volatiles From Intact and Mechanically Damaged Annual Bluegrass},
	journal = {Environmental Entomology},
	volume = {40},
	number = {2},
	year = {2011},
	month = {Apr},
	pages = {412-419},
	abstract = {Listronotus maculicollis Kirby is a highly destructive pest of low mown, cool-season turfgrasses in the northeastern United States and Canada. Behavioral and electrophysiological assays were conducted to identify compounds that may be useful in developing novel monitoring techniques. In Y-tube assays, males and females responded differently to volatiles from intact and clipped annual bluegrass (Poa annua L.). Females were significantly attracted to intact P. annua but repelled from clippings; males did not respond significantly to either treatment. Electroantennogram (EAG) recordings from both sexes showed a significant response to volatiles from both treatments. Gas chromatography mass spectrometry (GC-MS) identified 12 volatile compounds from P. annua of which nine were common to both intact plants and clippings. On average, seven-fold higher quantities of volatiles were collected from clippings than from intact plants (24.3 versus 3.4 ng/g of tissue/h). Eight compounds were released in significantly greater quantities from clippings of which 50\% were the n-C6 compounds hexanal, (E)-2-hexenal, and (Z)-3-hexenyl acetate, commonly referred to as "green leaf volatiles" (GLVs). Only octanal was emitted in greater amounts from intact plants than clippings. These nine compounds were tested individually against male and female antennae. Both sexes displayed greatest sensitivity to nonanal, octanal, and (E)-2-hexenal, but a significant dose-D response relationship was observed with all compounds tested. These studies indicate that both sexes respond physiologically and that L. maculicollis females exhibit behavioral responses to host-plant volatiles. Future studies will need to assess the effects of individual compounds and component mixtures on L. maculicollis behavior in the field.},
	keywords = {Listronotus maculicollis},
	isbn = {0046-225X},
	author = {McGraw, Benjamin A. and Rodriguez-Saona, Cesar and Holdcraft, Robert and Szendrei, Zsofia and Koppenhoefer, Albrecht M.}
}
